Coding

Part:BBa_K1112001:Design

Designed by: Alba Iglesias Vilches   Group: iGEM13_Valencia_Biocampus   (2013-09-10)


pGlnA + hmsHFRS operon


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 950
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2078
    Illegal BamHI site found at 5434
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 298
    Illegal NgoMIV site found at 472
    Illegal NgoMIV site found at 1966
    Illegal NgoMIV site found at 2374
    Illegal NgoMIV site found at 2380
    Illegal NgoMIV site found at 2470
    Illegal NgoMIV site found at 2683
    Illegal AgeI site found at 3040
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 543


Design Notes

We developed a nitrogen-gradation tube experiment, which means that we added different (NH4)2SO4 concentrations to each tube, so we can measure a gradation of the protein fluorescence. We used a synthetic medium for this.


Source

The operon used comes from Xenorhabdus nematophila, a nematode-dependent parasite. This operon allows the formation of a biofilm on nematodes pathogenic to insects and it is thus key in this nematode-bacteria symbiotic association that kills insects.

References

1. Drace K, Darby C. The hmsHFRS operon of Xenorhabdus nematophila is required for biofilm attachment to Caenorhabditis elegans. Appl Environ Microbiol. 2008 Jul;74(14):4509-15. doi: 10.1128/AEM.00336-08. Epub 2008 May 30.

2. Darby C, Hsu JW, Ghori N, Falkow S. Caenorhabditis elegans: plague bacteria biofilm blocks food intake. Nature. 2002 May 16;417(6886):243-4.